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Detection and genome characterization of <i>Potato virus</i> Y isolates infecting potato (<i>Solanum tuberosum</i> L.) in La Union (Antioquia, Colombia)

Gutiérrez S., Pablo and Marín M., Mauricio and Muñoz E., Daniel (2016) Detection and genome characterization of <i>Potato virus</i> Y isolates infecting potato (<i>Solanum tuberosum</i> L.) in La Union (Antioquia, Colombia). Agronomía Colombiana, 34 (3). pp. 317-328. ISSN 2357-3732

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Resumen

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

Tipo de documento:Artículo - Article
Palabras clave:ELISA, genomics, plant viruses, potyviruses, variants
Temática:6 Tecnología (ciencias aplicadas) / Technology > 63 Agricultura y tecnologías relacionadas / Agriculture
Unidad administrativa:Revistas electrónicas UN > Agronomía Colombiana
Código ID:60219
Enviado por : Dirección Nacional de Bibliotecas STECNICO
Enviado el día :28 Noviembre 2017 15:48
Ultima modificación:28 Noviembre 2017 15:48
Ultima modificación:28 Noviembre 2017 15:48
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