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Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.)

Murillo-Gómez, Paola Andrea and Hoyos S, Rodrigo and Chavarriaga, Paul (2017) Organogenesis in-vitro using three tissues types of tree tomato (<i>Cyphomandra betacea</i> (Cav.)) (Sendtn.). Agronomía Colombiana, 35 (1). pp. 5-11. ISSN 2357-3732

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Resumen

Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction of organogenesis in vitro using three different tissues: leaves, petioles and sexual seeds of the variety Common. All tissues were cultured on MS supplemented with agar, sucrose and TDZ or BAP phytohormones at 0.5 to 3 mg L-1, combined or not with the auxins IAA and NAA. Although petioles and seeds had a great potential for regeneration of plantlets, leaves produced more shoots (average of 18.4 shoots/explant) on medium containing TDZ 0.5 mg L-1. Additionally, its easy handling and resistance to physical damage may be useful traits to perform genetic transformation. The highest average of shoots produced from seeds and petioles were 4.3 and 3.1 shoots/explant, respectively. The production of multiple shoots in petioles was influenced by low concentrations of auxins. The emergence of the radicle in the seeds was important for the formation of shoots.

Tipo de documento:Artículo - Article
Palabras clave:Clonal propagation, plant regeneration, shoot induction, tamarillo, tissue culture
Temática:6 Tecnología (ciencias aplicadas) / Technology > 63 Agricultura y tecnologías relacionadas / Agriculture
Unidad administrativa:Revistas electrónicas UN > Agronomía Colombiana
Código ID:60209
Enviado por : Dirección Nacional de Bibliotecas STECNICO
Enviado el día :28 Noviembre 2017 15:48
Ultima modificación:28 Noviembre 2017 15:48
Ultima modificación:28 Noviembre 2017 15:48
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